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Clinical Chemistry 46: 1415-1416, 2000;
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(Clinical Chemistry. 2000;46:1415-1416.)
© 2000 American Association for Clinical Chemistry, Inc.

Technical Briefs

Sex Hormone-binding Globulin Concentration: Differences Among Commercially Available Methods

Charlotte Bukowski1, Mary Ann Grigg1 and Christopher Longcope1,2,a

1 Departments of Obstetrics and Gynecology and
2 Medicine, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655
a address correspondence to this author at: Department of OB/GYN, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655

Sex hormone-binding globulin (SHBG) is a protein that binds certain androgens and estrogens with high affinity (1)(2) and restricts the access of such steroids to tissues as long as the steroids remain bound to SHBG (3). Measurements of SHBG are often done on a clinical basis and can be used to determine the amounts of free and bioavailable estradiol and testosterone (3)(4)(5). Initially, the measurements were indirect (6)(7)(8)(9), but radioimmunoassays were developed (10)(11). Now several commercial methods are available to measure SHBG directly using the interaction of SHBG with an antibody. We have found marked differences in the values obtained by these methods.

Blood samples were obtained from 20 men (ages 21–30 years) and 20 women (ages 21–42 years) between 0800 and 1000. The men and women were healthy by history and a brief physical exam, and were taking no medications. The women all had regular menstrual cycles. The samples were allowed to clot, and the serum was collected, divided into aliquots, and stored at -80 °C until analyzed. Analyses were carried out within 3 months of blood collection. The protocol was . . . [Full Text of this Article]


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The following articles in journals at HighWire Press have cited this article:


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K. Sorensen, A. M. Andersson, N. E. Skakkebaek, and A. Juul
Serum Sex Hormone-Binding Globulin Levels in Healthy Children and Girls with Precocious Puberty before and during Gonadotropin-Releasing Hormone Agonist Treatment
J. Clin. Endocrinol. Metab., August 1, 2007; 92(8): 3189 - 3196.
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W. de Ronde, Y. T. van der Schouw, H. A.P. Pols, L. J.G. Gooren, M. Muller, D. E. Grobbee, and F. H. de Jong
Calculation of Bioavailable and Free Testosterone in Men: A Comparison of 5 Published Algorithms
Clin. Chem., September 1, 2006; 52(9): 1777 - 1784.
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A. M. Matsumoto and W. J. Bremner
Serum Testosterone Assays--Accuracy Matters
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 520 - 524.
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