Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 370-372, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Török, D.
Right arrow Articles by Waldhauser, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Török, D.
Right arrow Articles by Waldhauser, F.
Related Collections
Right arrow Molecular Diagnostics and Genetics
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 2002;48:370-372.)
© 2002 American Association for Clinical Chemistry, Inc.

Technical Briefs

Stability of 17{alpha}-Hydroxyprogesterone in Dried Blood Spots after Autoclaving and Prolonged Storage

Dóra Török1,2, Adolf Mühl1, Felix Votava3, Georg Heinze4, János Sólyom2, Julia Crone1, Sylvia Stöckler-Ipsiroglu1 and Franz Waldhauser1a

Departments of
1 Pediatrics and
4 Medical Computer Sciences, University of Vienna, A-1090 Vienna, Austria
2 2nd Department of Pediatrics, Semmelweis University, H-1094 Budapest, Hungary

3 Department of Pediatrics, Charles University, CZ-10081 Prague, Czech Republic

aaddress correspondence to this author at: Department of Pediatrics, University of Vienna, AKH-Wien, Währinger Gürtel 18-20, A-1090 Vienna, Austria; fax 43-1-40-400-3238, e-mail franz.waldhauser@akh-wien.ac.at

In the last few decades, management of patients with inborn errors of metabolism or congenital endocrine disorders has been greatly improved by the introduction of neonatal mass screening programs in many parts of the world. For this purpose, blood from newborns, ideally 4–7 days of age, is collected on filter-paper cards by means of a heel prick. A standard set of up to 20 tests is usually carried out at a centralized laboratory within a few days. Until recently, either when the tests were being performed or during the final step, the entire filter-paper card was autoclaved and then stored (1). Thus, leftover blood spots on filter paper from the entire young population in many countries are available in native or autoclaved form, although autoclaving has now become outdated because of a change in analytical methods (1). The stored cards provide a potentially valuable source for population studies or retrospective examinations. Documentation of the long-term stability of the analytes to be examined is a prerequisite for such studies.

In many countries, the latest addition to the neonatal screening program is the examination for congenital adrenal hyperplasia (CAH), by measurement of 17{alpha}-hydroxyprogesterone (17-OHP) in a dried blood spot (2)(3)(4). The technique, first described by Pang et al. in 1977 (5), is based on a direct immunoassay and is used as the standard procedure in programs screening for CAH (2). In several trials, 17-OHP in dried blood spots on filter-paper cards was resistant to freezing, high ambient temperatures, humidity, and short- or intermediate-term (up to 1 year) storage (6)(7)(8)(9)(10). In the present study, we examined the impact of autoclaving (i.e., extremely high temperature, high humidity and pressure) . . . [Full Text of this Article]


Acknowledgments


References




The following articles in journals at HighWire Press have cited this article:


Home page
 J Med ScreenHome page
V. S Lando, M. C Batista, I. T Nakamura, C. R Mazi, B. B Mendonca, and V. N Brito
Effects of long-term storage of filter paper blood samples on neonatal thyroid stimulating hormone, thyroxin and 17-alpha-hydroxyprogesterone measurements
J Med Screen, September 1, 2008; 15(3): 109 - 111.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
K. A. Strnadova, M. Holub, A. Muhl, G. Heinze, R. Ratschmann, H. Mascher, S. Stockler-Ipsiroglu, F. Waldhauser, F. Votava, J. Lebl, et al.
Long-Term Stability of Amino Acids and Acylcarnitines in Dried Blood Spots
Clin. Chem., April 1, 2007; 53(4): 717 - 722.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
F. Votava, D. Torok, J. Kovacs, D. Moslinger, S. M Baumgartner-Parzer, J. Solyom, Z. Pribilincova, T. Battelino, J. Lebl, H. Frisch, et al.
Estimation of the false-negative rate in newborn screening for congenital adrenal hyperplasia
Eur. J. Endocrinol., June 1, 2005; 152(6): 869 - 874.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.