Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 581-583, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lynch, P. L. M.
Right arrow Articles by O’Kane, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lynch, P. L. M.
Right arrow Articles by O’Kane, M. J.
Related Collections
Right arrow General Clinical Chemistry
Right arrow Endocrinology and Metabolism
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 2002;48:581-583.)
© 2002 American Association for Clinical Chemistry, Inc.

Technical Briefs

Methemoglobin Interferes with the HemoCue B-Glucose Analyzer

Patrick L. M. Lynch1a and Maurice J. O’Kane1

1 Department of Clinical Chemistry, Altnagelvin Area Hospital, Glenshane Rd., Londonderry BT47 6SB, United Kingdom

aauthor for correspondence: fax 44-2871-313036, e-mail mlynch@alt.n-i.nhs.uk

During the investigation of a neonate with NADH-methemoglobin (NADH-MetHb) reductase deficiency (baseline MetHb, 28%), we noticed disparate readings between the ward-based, whole-blood HemoCue B-Glucose Analyzer (HemoCue AB) and the main laboratory plasma glucose method (glucose oxidase) performed on a Synchron CX7 Delta analyzer (Beckman Coulter). The HemoCue results suggested profound hypoglycemia, which was out of keeping with the clinical status of the patient, whereas the Synchron CX7 Delta results were consistently within the pediatric reference interval. These findings were contrary to the previously documented overestimation of the HemoCue analyzer at low glucose concentrations (1)(2)(3). Because the HemoCue analyzer (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) uses a mutarose–glucose dehydrogenase enzyme system to produce a colored formazan, which is then quantified photometrically, the possibility of spectral interference by MetHb with the HemoCue analyzer was raised . . . [Full Text of this Article]


References




The following articles in journals at HighWire Press have cited this article:


Home page
 Diabetes CareHome page
K. Dungan, J. Chapman, S. S. Braithwaite, and J. Buse
Glucose Measurement: Confounding Issues in Setting Targets for Inpatient Management
Diabetes Care, February 1, 2007; 30(2): 403 - 409.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.