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Technical Briefs |
1 Department of Biochemistry and Hormonology, Hôpital Antoine Béclère, 157 rue de la Porte de Trivaux, 92141 Clamart cedex, France
aauthor for correspondence: fax 33-1-45374745, e-mail joelle.taieb@abc.ap-hop-paris.fr
Rapid steroid hormone immunoassays often agree poorly, especially at normal and low concentrations (1)(2)(3)(4). These problems result from low assay specificity, inadequate standardization, and poor optimization of the methods over the large range of concentrations seen clinically (5)(6)(7). These systems are often unsuitable for clinical applications that require a low detection limit, such as the following: (a) estradiol measurements in men [<110 pmol/L; (<30 pg/mL)] or children [from <18 pmol/L to 165 pmol/L (<5 pg/mL to 45 pg/mL)] (8) and evaluation of down-regulation by gonadoliberin analogs before in vitro fertilization and embryo transfer (IVF-ET) programs; (b) progesterone determinations during ovarian stimulation, with values <3.2 nmol/L (<1 ng/mL) on the day of human chorionic gonadotropin administration predictive for pregnancy in IVF-ET (9)(10); (c) testosterone assays for children [from <0.35 nmol/L to 5 nmol/L (<0.1 ng/mL to 1.5 ng/mL)] and women [<2.4 nmol/L (<0.7 ng/mL)] (11)(12). Furthermore, limits of detection determined with the zero calibrator are generally far below the lowest concentration that can be reliably quantified in human serum [functional sensitivity (13)(14) or limit of quantitation (LOQ) (15)].
In this study, we analyzed and compared detection limits and functional sensitivities for nine estradiol (E2) and eight progesterone (P) immunoassays.
Between 1997 and 2001, we tested nine
Acknowledgments
References
The following articles in journals at HighWire Press have cited this article:
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  G. A Wood, J. E Fata, K. L M Watson, and R. Khokha Circulating hormones and estrous stage predict cellular and stromal remodeling in murine uterus Reproduction, May 1, 2007; 133(5): 1035 - 1044. [Abstract] [Full Text] [PDF]
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 E. Orwoll, L. C. Lambert, L. M. Marshall, J. Blank, E. Barrett-Connor, J. Cauley, K. Ensrud, S. R. Cummings, and for the Osteoporotic Fractures in Men Study Group Endogenous testosterone levels, physical performance, and fall risk in older men. Arch Intern Med, October 23, 2006; 166(19): 2124 - 2131. [Abstract] [Full Text] [PDF]
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 M. M. Kushnir, A. L. Rockwood, W. L. Roberts, E. G. Pattison, W. E. Owen, A. M. Bunker, and A. W. Meikle Development and Performance Evaluation of a Tandem Mass Spectrometry Assay for 4 Adrenal Steroids Clin. Chem., August 1, 2006; 52(8): 1559 - 1567. [Abstract] [Full Text] [PDF]
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 J. P. Bilezikian What's Good for the Goose's Skeleton is Good for the Gander's Skeleton. J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1223 - 1225. [Full Text] [PDF]
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E. Orwoll, L. C. Lambert, L. M. Marshall, K. Phipps, J. Blank, E. Barrett-Connor, J. Cauley, K. Ensrud, S. Cummings, and for the Osteoporotic Fractures in Men Study Group Testosterone and Estradiol among Older Men J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1336 - 1344. [Abstract] [Full Text] [PDF]
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M. M. Kushnir, A. L. Rockwood, W. L. Roberts, E. G. Pattison, A. M. Bunker, R. L. Fitzgerald, and A. W. Meikle Performance Characteristics of a Novel Tandem Mass Spectrometry Assay For Serum Testosterone Clin. Chem., January 1, 2006; 52(1): 120 - 128. [Abstract] [Full Text] [PDF]
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Z. Cao, T. A. Swift, C. A. West, T. G. Rosano, and R. Rej Immunoassay of Estradiol: Unanticipated Suppression by Unconjugated Estriol Clin. Chem., January 1, 2004; 50(1): 160 - 165. [Abstract] [Full Text] [PDF]
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J. Taieb, C. Benattar, R. Diop, A. S. Birr, and A. Lindenbaum Use of the Architect-i2000 Estradiol Immunoassay during in Vitro Fertilization Clin. Chem., January 1, 2003; 49(1): 183 - 186. [Full Text] [PDF]
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