Clinical Chemistry Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 679-680, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Costa, J.-M.
Right arrow Articles by Ernault, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Costa, J.-M.
Right arrow Articles by Ernault, P.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 2002;48:679-680.)
© 2002 American Association for Clinical Chemistry, Inc.

Letters

Automated Assay for Fetal DNA Analysis in Maternal Serum

Jean-Marc Costa1a and Pauline Ernault1

1 M. Dassault Molecular Biology Laboratory, Centre de Diagnostic Prénatal, American Hospital of Paris, 63 bd Victor Hugo, 92200 Neuilly-sur-Seine, France

aAuthor for correspondence. E-mail jean-marc.costa@ahparis.org.


To the Editor:

Since the first demonstration of the presence of cell-free fetal DNA in maternal plasma of pregnant women (1), many reports have been published, mainly about fetal sex and RhD determinations (2)(3). Although good results have been obtained with conventional PCR, real-time PCR is now the most widely used amplification system for fetal DNA analysis because it allows high sensitivity with a high degree of protection against contamination (4). Conventional PCR may produce false-positive results from contamination (5), whereas real-time PCR, because it is a closed-tube system, reduces the risk of false-positive results from carryover of PCR products (6)(7). During sample preparation, however, contamination can result either from cross-contamination between samples or from the operator, particularly if a very small amount of human target sequence has to be detected, such as cell-free fetal DNA in . . . [Full Text of this Article]


Acknowledgments


References




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. W.K. Chiu, W.-b. Lui, A. El-Sheikhah, A. T.C. Chan, T. K. Lau, K. H. Nicolaides, and Y.M. D. Lo
Comparison of Protocols for Extracting Circulating DNA and RNA from Maternal Plasma
Clin. Chem., November 1, 2005; 51(11): 2209 - 2210.
[Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
Y.M. D. Lo
Recent Advances in Fetal Nucleic Acids in Maternal Plasma
J. Histochem. Cytochem., March 1, 2005; 53(3): 293 - 296.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Guibert, A. Benachi, A.-G. Grebille, P. Ernault, J.-R. Zorn, and J.-M. Costa
Kinetics of SRY gene appearance in maternal serum: detection by real time PCR in early pregnancy after assisted reproductive technique
Hum. Reprod., August 1, 2003; 18(8): 1733 - 1736.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. J. Johnson and Y.M. D. Lo
Plasma Nucleic Acids in the Diagnosis and Management of Malignant Disease
Clin. Chem., August 1, 2002; 48(8): 1186 - 1193.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.