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Clinical Chemistry 48: 1600-1601, 2002;
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(Clinical Chemistry. 2002;48:1600-1601.)
© 2002 American Association for Clinical Chemistry, Inc.

Technical Briefs

Detection of Monoclonal Proteins in Sera by Capillary Zone Electrophoresis and Free Light Chain Measurements

Godelieve Mariën1, Els Oris1, Arthur R. Bradwell2, Norbert Blanckaert1 and Xavier Bossuyt1a

1 Laboratory Medicine, Immunology, University Hospitals Leuven, Herestraat 49, B-3000 Leuven, Belgium;
2 Department of Immunology, University of Birmingham, The Medical School, Birmingham B15 2TJ, United Kingdom

aaddress correspondence to this author at: Laboratoriumgeneeskunde, Immunologie, Universitaire Ziekenhuizen Leuven, Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium; fax 32-16-347-931, e-mail Xavier.bossuyt@uz.kuleuven.ac.be

The first 20% of the full text of this article appears below.

Capillary zone electrophoresis (CZE) is increasingly used in clinical laboratories for serum protein separation. The technique is a sensitive method for the detection of monoclonal (M) proteins. However, when compared with immunofixation, CZE fails to detect M proteins in 5% of samples (1)(2). These so-called "false-negative" results encompass low-concentration and "hidden" M proteins (e.g., in the transferrin peak).

The goal of this study was to evaluate whether an assay for the detection of free light chains (FLCs) that has recently become available (Freelite®; The Binding Site) can detect the M proteins present in samples that produce false-negative results in CZE. The Freelite assay is a sensitive automated immunoassay for {kappa} and {lambda} FLCs in serum (3) and has been useful for the diagnosis and monitoring of patients with nonsecretory myeloma (4). Moreover, it has been reported that the quantification of FLCs in serum by nephelometry correlates with changes in urinary FLC excretion (5).

Frozen sera from 54 patients that had previously been shown to contain M . . . [Full Text of this Article]






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