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Technical Briefs |
Departments of1 Chemical Pathology,2 Paediatrics, 4 Medicine and Therapeutics, and5 Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR;3 Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region
aaddress correspondence to this author at: Department of Chemical Pathology, The Chinese University of Hong Kong, Room 38023, 1/F Clinical Sciences Building, Prince of Wales Hospital, 30--32 Ngan Shing St., Shatin, New Territories, Hong Kong Special Administrative Region, China; e-mail loym@cuhk.edu.hk
| The first 300 words of the full text of this article appear below. |
The recent identification of a novel coronavirus, SARS coronavirus (SARS-CoV), as an etiologic agent for severe acute respiratory syndrome (SARS) has prompted many groups to develop rapid and accurate molecular assays for the detection of this virus (1)(2)(3)(4). To date, most of the assays have focused predominantly on samples taken from nasopharyngeal aspirates, urine, and stools (5)(6). Although detection of SARS-CoV RNA in the plasma of SARS patients has been reported (1), the relatively low sensitivity of the ultracentrifugation-based approach for detecting SARS-CoV RNA in plasma has made this assay impractical. Recently we showed that a one-step real-time quantitative reverse transcription-PCR (RT-PCR) assay for the polymerase region of the SARS-CoV genome could detect viral RNA in 75–78% of nonultracentrifuged serum samples from adult SARS patients during the early stage of disease and that the serum SARS-CoV concentrations on admission were of prognostic significance (7). This finding demonstrates that plasma/serum SARS-CoV quantification may potentially be useful for the early diagnosis of SARS.
Although most existing reports have focused on adult SARS patients, recent reports revealed that the clinical course was less severe in pediatric SARS patients than in adult SARS patients (8)(9). On the whole, the outcomes of pediatric SARS patients have been favorable. In this study, we investigated whether SARS-CoV RNA can be detected in the plasma samples of pediatric patients during different stages of SARS and studied the serial variation in viral loads.
We quantified SARS-CoV RNA by real-time RT-PCR in the plasma of eight pediatric patients admitted to the New Territories East Cluster of Hospital Authority Hospitals in Hong Kong and who satisfied the WHO surveillance case definition for SARS (9). These patients were recruited between
The following articles in journals at HighWire Press have cited this article:
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  A. Bitnun, S. Read, R. Tellier, M. Petric, and S. E. Richardson Severe Acute Respiratory Syndrome-Associated Coronavirus Infection in Toronto Children: A Second Look Pediatrics, January 1, 2009; 123(1): 97 - 101. [Abstract] [Full Text] [PDF]
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 A M Li and P C Ng Severe acute respiratory syndrome (SARS) in neonates and children Arch. Dis. Child. Fetal Neonatal Ed., November 1, 2005; 90(6): F461 - F465. [Abstract] [Full Text] [PDF]
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 F W T Cheng, P C Ng, W K Chiu, W C W Chu, A M Li, K L Lo, E K L Hon, E A S Nelson, T F Leung, W H Ng, et al. A case-control study of SARS versus community acquired pneumonia Arch. Dis. Child., July 1, 2005; 90(7): 747 - 749. [Abstract] [Full Text] [PDF]
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W.-K. Wang, C.-T. Fang, H.-L. Chen, C.-F. Yang, Y.-C. Chen, M.-L. Chen, S.-Y. Chen, J.-Y. Yang, J.-H. Lin, P.-C. Yang, et al. Detection of Severe Acute Respiratory Syndrome Coronavirus RNA in Plasma during the Course of Infection J. Clin. Microbiol., February 1, 2005; 43(2): 962 - 965. [Abstract] [Full Text] [PDF]
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 T. C.W. Poon, K.C. A. Chan, P.-C. Ng, R. W.K. Chiu, I. L. Ang, Y.-k. Tong, E. K.O. Ng, F. W.T. Cheng, A. M. Li, E. K.L. Hon, et al. Serial Analysis of Plasma Proteomic Signatures in Pediatric Patients with Severe Acute Respiratory Syndrome and Correlation with Viral Load Clin. Chem., August 1, 2004; 50(8): 1452 - 1455. [Full Text] [PDF]
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 D S C Hui, M C H Chan, A K Wu, and P C Ng Severe acute respiratory syndrome (SARS): epidemiology and clinical features Postgrad. Med. J., July 1, 2004; 80(945): 373 - 381. [Abstract] [Full Text] [PDF]
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L. L.M. Poon, C. S.W. Leung, M. Tashiro, K. H. Chan, B. W.Y. Wong, K. Y. Yuen, Y. Guan, and J. S.M. Peiris Rapid Detection of the Severe Acute Respiratory Syndrome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay Clin. Chem., June 1, 2004; 50(6): 1050 - 1052. [Full Text] [PDF]
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