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Clinical Chemistry 49: 676-678, 2003; 10.1373/49.4.676
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(Clinical Chemistry. 2003;49:676-678.)
© 2003 American Association for Clinical Chemistry, Inc.

Technical Briefs

Measurement of ß1- and ß2-Globulins Improves Detection of M-Spikes on High-Resolution Electrophoresis

Srinivas Narayan1, M. Giovanni Lujan1, Leland B. Baskin1, Sridevi Devaraj3, Cynthia Rutherford2 and Ishwarlal Jialal3,a

Departments of
1 Pathology and
2 Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75390
3 Department of Pathology, University of California Davis Medical Center, 4635 2nd Ave., Room 3000, Sacramento, CA 95817

aauthor for correspondence: fax 916-734-6593, e-mail ishwarlal.jialal@ucdmc.ucdavis.edu

The first 20% of the full text of this article appears below.

High-resolution serum protein electrophoresis (SPE) provides a clearer separation of ß1- and ß2-globulins than does low-resolution SPE. Currently, immunofixation electrophoresis (IFE) is performed in most laboratories based on high clinical suspicion of a disease associated with a monoclonal gammopathy or an abnormality in the pattern displayed on the electrophoretogram. The aim of this study was to prospectively determine the utility of performing IFE on specimens with increased ß1- or ß2-globulins on high-resolution SPE but with a normal electrophoretic pattern on visual inspection.

SPE was performed with a Sebia-Hydrasys automated electrophoresis system (Hydragel ß-1 + ß-2 gels; Sebia). The same system was used for IFE with polyclonal anti-human serum for identifying immunoglobulin heavy and light chains. The reference intervals for ß1 and ß2 concentrations, determined from 100 fresh samples from healthy volunteers in this laboratory, were 4–8 and 1–5 g/L (mean ± 2 SD), respectively.

This study received approval from our Institutional Review Board. During the period from March to December 2001, 3179 samples were submitted for SPE. Of these, 963 had IFE performed because of clinical suspicion or abnormalities in the SPE. All serum specimens submitted for SPE with increased ß1 or ß2 (n = 51) were prospectively evaluated by IFE. Fifteen of the samples had an obvious M-spike that warranted an IFE and were excluded from the analyses. As a control group, 50 samples with protein concentrations within the reference intervals and a visually normal electrophoretogram were also subjected to IFE. Criteria for inclusion were (a) no distinct protein band evident outside of the usual six bands (albumin, {alpha}1, {alpha}2, ß1, ß2, and {gamma}) and (b) an increased ß1 or ß2 concentration. LDL-cholesterol, . . . [Full Text of this Article]






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