Clinical Chemistry
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Clinical Chemistry 49: 1029-1030, 2003; 10.1373/49.6.1029
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(Clinical Chemistry. 2003;49:1029-1030.)
© 2003 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Automated Enzymatic Assay for Homocysteine

Yuying Tan1, Xinghua Sun1, Li Tang1, Nan Zhang1, Qinghong Han1, Mingxu Xu1, Xuezhong Tan1, Xiuying Tan1 and Robert M. Hoffman1,a

1 A/C Diagnostics, LLC, and AntiCancer, Inc., 7917 Ostrow St. San Diego, CA 92111

aAuthor for correspondence. Fax 858-268-4175; e-mail all@anticancer.com.

The first 20% of the full text of this article appears below.


To the Editor:

Various methods have been developed for plasma total homocysteine (tHCY) measurement, including a tHCY enzyme conversion immunoassay designed for the Abbott IMx analyzer (1), a microtiter plate tHCY enzymatic immunoassay assay (2), HPLC methods (3)(4)(5), and gas chromatography–mass spectrometry methods (6). We have described a single-enzyme tHCY assay (enzymatic tHCY assay) based on a highly specific recombinant form of L-homocysteine {alpha},{gamma}-lyase (rHCYase) (7)(8). We report here the application of this tHCY enzymatic assay on the Hitachi 912 automatic chemistry analyzer. The principle of the assay is that rHCYase produces H2S from tHCY and that the H2S is quantified by its reaction with N,N-dibutylphenylenediamine, which produces a chromophore.

The assay uses four . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
Q. Han, X. Sun, M. Xu, N. Zhang, L. Tang, Y. Tan, and R. M. Hoffman
3-Deazaadenosine, a Stabilizer of Whole-Blood Homocysteine Content, Does Not Interfere with the Single-Enzyme Homocysteine Assay while Totally Inhibiting the Enzyme Conversion Homocysteine Immunoassay
Clin. Chem., September 1, 2004; 50(9): 1703 - 1704.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
Q. Han, M. Xu, L. Tang, X. Sun, N. Zhang, X. Tan, X. Tan, Y. Tan, and R. M. Hoffman
Homogeneous Enzymatic Colorimetric Assay for Total Cysteine
Clin. Chem., July 1, 2004; 50(7): 1229 - 1231.
[Full Text] [PDF]


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