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Clinical Chemistry 49: 1419-1421, 2003; 10.1373/49.8.1419
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(Clinical Chemistry. 2003;49:1419-1421.)
© 2003 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Clinical Capillary Zone Electrophoresis of Serum Proteins: Balancing High Sensitivity and High Specificity

Godelieve Mariën1, Guido Vranken2, Michel Demuylder2, Norbert Blanckaert1 and Xavier Bossuyt1,a

1 Laboratory Medicine, Immunology, University Hospital Leuven, Herestraat 49, B-3000 Leuven, Belgium

2 Analis Inc., Rue Dewez 14, B-5000 Namur, Belgium

aAuthor for correspondence. Fax 32-16-34-7931; e-mail Xavier.Bossuyt@uz.kuleuven.ac.be.

The first 20% of the full text of this article appears below.


To the Editor:

Capillary zone electrophoresis (CZE) is documented to have high sensitivity and specificity for the detection of monoclonal (M) proteins. In two large (>1500 participants) prospective studies in which CZE was compared with "immunofixation/immunoelectrophoresis", the sensitivity of CZE was 95% (1)(2). This is superior to the sensitivity of agarose gel electrophoresis (AGE), which Katzmann et al. (1) found to be 91% and Meunier (3) found to be 92.2% (evaluation of 2060 sera containing an M-protein). Especially in the ß-globulin region, CZE with Paragon CZE 2000 (Beckman-Coulter) is superior to AGE for detection of low-concentration M-proteins (IgA and light chains) (3)(4). The specificity of CZE is reported to be high (98.6%) and comparable to that of AGE (1)(5).

In a prospective study (2), we described three M-proteins with high pI values that migrated in the slow {gamma} region on AGE but were not detected with Paragon. In addition, one high-concentration IgG|gl with a pI {approx}7 that migrated in the mid-{gamma} region on AGE was not separated by CZE. The Paragon has recently been upgraded [modified buffer, higher voltage (10.3 kV), more efficient . . . [Full Text of this Article]

Kevin Day3 and Jack Zakowski3,b

3 Beckman Coulter, Inc., 200 S. Kraemer Blvd., Brea, CA 92822

bAuthor for correspondence.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
X. Bossuyt and G. Marien
Detection of Monoclonal Proteins by Capillary Zone Electrophoresis: Comparison of 2 Multichannel Automated Systems
Clin. Chem., January 1, 2007; 53(1): 152 - 153.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
K. Gijbels, G. Marien, and X. Bossuyt
Ethanol Precipitation Is Not Reliable for Selectively Removing Nonmonoclonal Peaks Seen in the Fibrinogen Region on Capillary Zone Electrophoresis of Serum Proteins
Clin. Chem., October 1, 2004; 50(10): 1880 - 1881.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. Marien and X. Bossuyt
Response to the Comments of K. Day and J. Zakowski on "Clinical Capillary Zone Electrophoresis of Serum Proteins: Balancing High Sensitivity and High Specificity"
Clin. Chem., October 1, 2003; 49(10): 1711 - 1712.
[Full Text] [PDF]


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