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San Diego Conference |
| The first 300 words of the full text of this article appear below. |
New Materials for Strongly Binding and Controllably Releasing Nucleic Acids.
H. Akhavan-Tafti, R. DeSilva, W.G. Cripps, R.A. Eickholt, R.S. Handley, K.S. Lauwers, M.D. Sandison, S. Siripurapu, and A.P. Schaap.
Lumigen Inc., Southfield, MI.
A new type of solid phase material for binding and releasing nucleic acids has been developed. The materials bind DNA and RNA with a strength far exceeding conventional materials and feature a novel mechanism to release bound nucleic acids. Unlike silica particle based materials and ion exchange polymeric resins, both of which elute bound nucleic acid through alteration of buffer ionic strength, neither high nor low ionic strength buffer is required to release nucleic acid. Bound nucleic acid is, however, readily released intact under controlled conditions with a 5 minute protocol. The solid phase materials have been fabricated as both magnetic and non-magnetic microparticles. No chaotropic salts are required to bind or release nucleic acids. DNA and RNA can be captured from water or a variety of buffers and media over a range of pH and salt concentrations. Binding of DNA and RNA from solution results in immobilization of nucleic acids that resists reversal under all commonly used release conditions. Repeated washing of the particles with water, low or high ionic strength buffers and surfactants does not remove captured nucleic acid. Nucleic acids of a wide range of molecular weight were isolated with the new materials. DNA ranging in size from oligonucleotides up to genomic fragments of several kilobases were captured quantitatively and released in high yield. Both magnetic and non-magnetic particles have been used in methods of isolating or purifying nucleic acids from several different sources. The particles were used to isolate the plasmid pUC18 from an overnight E. coli culture after pelleting, SDS lysis and potassium acetate precipitation. Human genomic DNA was extracted from whole blood with a conventional alkaline lysis procedure. DNA in the supernatants was captured on 10 mg of particles and released to produce DNA of MW 
20kb. In addition, a simple and rapid protocol
Diagnosis of Aneuploidies within 6 Hours Using Flow-Through PamChip Microarrays.
R. van Beuningen,1 L. Vahlkamp,1 M.E. Kalf,1,2 S.J.White,2 M.Kriek,2 T. den Dunnen,2 M.H. Breuning,2 and A. Chan.1
1 PamGene International B.V. ’s Hertogenbosch, The Netherlands; 2Leiden University Medical Center, Leiden, The Netherlands.
The NCI-Funded Standardized Expression Measurement (SEM) CenterTM Based on StaRT-PCRTM Enables Multi-Institutional Clinical Trials and Facilitates Development of Drugs and Molecular Diagnostic Tests.
Terry W. Osborn, James C. Willey, Charles R. Knight, Cheryl A. Motten, Bradley J. Austermiller, Elizabeth A. Herness Peters, Robert J. Zahorchak, and Andy Bass.
Medical College of Ohio, Toledo, OH and Gene Express, Inc., Toledo, OH.
Development of a Fully Automated Protocol of MagaZorb Nucleic Acid Isolation Reagents on PSS Magtration System 12GC.
Jagdish Saini, Nnamdi Uche, Shiva Pourkaveh, Dokhi Nargessi, Dan Maffeo, and Chris Kusumoto.
Cortex Biochem, San Leandro, CA.
Opportunity and Application of Innovated Quantitative Fluorescent PCR (IQF PCR) in Fetal DNA Analysis in Non-Invasive Prenatal Diagnosis.
Radek Vodicka,1 Radek Vrtel,1 Ladislav Dusek,2 Dita Svabova,1 and Jiri Santavy.1
1Department of Clinical Genetics and Fetal Medicine, University Hospital Olomouc, Czech Republic; 2Centre of Biostatistics and Analyses, Masasyk University, Brno, Czech Republic.
Appearance of a Novel Antibody Against Insulin Activated Nitric Oxide Synthase (IANOS) in the Circulation in Acute Coronary Syndrome (ACS).
U. Ray,1,3G. Khan, K. Chakraborty,1 S. Chakraborty, G. Reddy, and A.K. Sinha.1,2
1Sinha Institute of Medical Science & Technology, Calcutta, India; 2Mount Sinai Medical Center, NY; 3Royal Hobart Hospital Hobart, Tasmania, Australia.
Stabilization of IFNß-Induced MxA Transcript in Post-Phlebotomy Whole Blood Using the PAXgeneTM Blood RNA System.
Lynne Rainen,1 Cynthia Ballas,2 Donna Dail,3 and Andrew Pachner.3
1PreAnalytiX Hombrechtikon, CH; 2BD, Franklin Lakes, NJ; 3University of Medicine and Dentistry, Newark, NJ.
Stabilization of Six Inflammatory Gene Transcripts in Post-Phlebotomy Whole Blood Using the PAXgeneTM Blood RNA System.
Lynne Rainen,1 Cynthia Ballas,2 David Trollinger,3 Danute Bankaitis-Davis,3 Shawn Lewis,3 David Gesink,3 and Michael Bevilacqua.3
1PreAnalytiX Hombrechtikon, CH; 2BD, Franklin Lakes, NJ; 3Source Precision Medicine, Boulder, CO.
A Single-Tube Extraction, Amplification, and Detection Method for Real Time PCR Using Nanoporous Aluminum Oxide Membranes.
Shale Dames,1 Marc Elgort,1 Mark Herrmann,1 Jacob Durtschi,1 L. Kathryn Bromley,1 Maria Erali,1 Roger Smith,1,2 and Karl Voelkerding.1,2
1ARUP Laboratories, Salt Lake City, UT; 2University of Utah, Salt Lake City, UT.
Rapid Detection of Enterovirus and or HSV from a Single Patient Preparation: Implications for Improving Clinical Outcomes and Resource Allocations.
Brian Mariani, Beatrix Meltzer, and Lacy Fehrenback.
Genetics and IVF Institute, Fairfax, VA.
Multiplexed PCR/Microfluidics Chip Assay to Determine Copy Number of Parkin (Park2) Exons.
Brian Dukek, Matthew Farrer, and Dennis O’Kane.
Mayo Clinic, Rochester, MN.
Fluorescent SNP Genotyping by High-Resolution Melting Analysis without Probes.
Michael Liew, Mark Johnson, Robert Graham, Cindy Meadows, Maria Erali, Rong Mao, Elaine Lyon, and Carl Wittwer.
Institute of Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT.
Optimization of Immunoassays Utilizing Multiplexed Protein Arrays.
Luis Burzio, Jo Ann Kraycer, and Sam Conzone.
Schott North America, Duryea, PA.
Simultaneous Genotyping of 36 Candidate Markers for Acute Myocardial Infarction Using Luminex®100TM XMAP Technology.
Andre Arellano, Bin Wang, David Lew, Wing Lee, Bradford Young, Dov Shiffman, Mary Luke, John Kane, Marc Short, and Lance Bare.
Celera Diagnostics, Alameda, CA.
Detection of Trace Amounts of Mutant K-ras DNA by Peptide Nucleic Acid as Both PCR Clamp and Sensor Probe.
Chiuan-Chian Chiou and Ji-Dung Luo.
Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Tao-Yuan, Taiwan.
Thrombophilia NucleoSight DNA Chip.
Wesley Lindsey, Rob Jenison, Evelyn Woodruff, and Heather Avens.
ThermoElectron, Louisville, CO.
Application: Use of Unlabeled Oligonucleotide Probe System in Molecular Diagnostics.
Lan-Szu Chou.1 Andrew Lyon,1 Cindy Meadows,1 Elaine Lyon,1,2 and Carl Wittwer.1,2
1ARUP Laboratories, Salt Lake City, UT; 2University of Utah, Salt Lake City, UT.
HepCgen® HCV Genotyping for Treatment (by Real Time PCR).
R. Cross, S. Jewell, and W. Rosenberg.
HepCGen Ltd., Southampton General Hospital, Southampton, UK.
A Biochip for Rapid K-ras Mutation Screening in Ovarian Cancer.
Robert Zeillinger,1,3 Gerhild Fabjani,1,2 Gernot Kriegshaeuser,3 Andreas Schuetz,4 and Lothar Prix.4
1Medical University of Vienna, Department of Obstetrics and Gynecology, Molecular Oncology Group, Vienna, Austria; 2Ludwig Boltzmann Institute for Gynecology and Gynecologic Oncology, Vienna, Austria; 3Vienna Lab Labordiagnostika, GmbH, Vienna, Austria; 4Biofocus GmbH, Recklinghansen, Germany.
Detection of Catalase Gene Mutations in Hungarian Acatalasemia.
L. Góth, P. Rass, A. Pay, and M. Vitai.
University of Debrecen, Departments of Clinical Biochemistry, Molecular Pathology and Clinical Analytical Chemistry, Debrecen, Hungary.
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