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Clinical Chemistry 50: 2391-2392, 2004. First published September 23, 2004; 10.1373/clinchem.2004.039248
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(Clinical Chemistry. 2004;50:2391-2392.)
© 2004 American Association for Clinical Chemistry, Inc.

Technical Briefs

Roche IFCC Methods for Lactate Dehydrogenase Tested for Duplicate Errors with Greiner and Becton-Dickinson Lithium-Heparin and Greiner Serum Samples

Goce Dimeski1,a, Tony Badrick2, Robert Flatman2 and Barry Ormiston1

1 Department of Chemical Pathology, Queensland Health Pathology Service, Princess Alexandra Hospital, Woolloongabba, Queensland Australia
2 Department of Chemical Pathology, Sullivan and Nicolaides Pathology, Taringa, Queensland, Australia

aaddress correspondence to this author at: Department of Chemical Pathology, Queensland Health Pathology Service (QHPS), Princess Alexandra Hospital, Ipswich Road, Woolloongabba, Queensland 4102, Australia; fax 61-7-3240-7070, e-mail Goce_Dimeski@health.qld.gov.au

The first 20% of the full text of this article appears below.

The new Roche IFCC-recommended method for lactate dehydrogenase (LD) has been reported (1)(2) to produce duplicate errors with Becton Dickinson (BD) lithium-heparin tubes with plasma separators. Separate studies (3) of Sarstedt tubes showed no such errors. To overcome and minimize duplicate errors, Roche has modified the method to incorporate a predilution step. This modified method can be used only on dilutor systems (Modular P and Hitachi 911, 912, and 917), but not on nondilutor systems (Modular D and Hitachi 747 and 902; Roche). We tested the modified method for duplicate errors and tested lithium-heparin tubes (with plasma separator) from another major supplier for duplicate errors.

Blood samples were collected in three different tubes: Greiner Vacuette lithium-heparin tubes with plasma separator (prod. no. 455083); Greiner Vacuette serum tubes with plasma separator (prod. no. 455078); and BD Vacutainer PST II lithium-heparin tubes with plasma separator (prod. no. 367377). Three IFCC LD methods were set up on our Modular DP system: the Modular D, the Modular P IFCC method, and the Modular P IFCC modified method with predilution. The two LD methods on the Modular . . . [Full Text of this Article]






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