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Clinical Chemistry 50: 779-780, 2004; 10.1373/clinchem.2003.025064
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(Clinical Chemistry. 2004;50:779-780.)
© 2004 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Effect of Different Sample Types and Stability after Blood Collection of N-Terminal Pro-B-Type Natriuretic Peptide as Measured with Roche Elecsys System

Da-elene van der Merwe1,a, Rob Henley1, Gary Lane2, Rachel Field2, Michael Frenneaux2, Frank Dunstan3 and Ian McDowell1

Departments of1 Medical Biochemistry and Immunology,2 Cardiology, and3 Medical Statistics, University Hospital of Wales and University of Wales College of Medicine, Cardiff CF14 4XW, United Kingdom

aAuthor for correspondence. Fax 44-2920-74-8383; e-mail DaElene.VanDerMerwe@CardiffandVale.wales.nhs.uk.

The first 20% of the full text of this article appears below.


To the Editor:

N-Terminal pro-B-type natriuretic peptide (NT-proBNP; 76-amino acid peptide) is cosecreted with BNP and can be measured by automated immunoassay (Elecsys 2010; Roche UK). This is a fully automated electrochemiluminescence "sandwich" immunoassay based on capture (biotinylated) and detection (ruthenium) polyclonal antibodies against NT-proBNP amino acids 1–21 and 39–50 (1), respectively. Reports on in vitro stability are conflicting (2)(3)(4). We assessed the effect of different sample types on measurement of NT-proBNP and evaluated the effect of delayed separation of serum from cells on stability. We compared plasma in lithium heparinate (14 IU/mL of blood; Greiner Labortechnik; cat no. 9045-22-1) and serum in serum separator (SST) tubes from patients attending a heart failure clinic. For sample type comparison, blood from 60 patients was collected into SST and lithium heparin tubes. Blood was also collected in . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Ann Clin BiochemHome page
B Cauliez, J Guignery, S Marinier, I Mariau, and A Lavoinne
Two-year stability of NT-proBNP in frozen samples using the Roche Elecsys system
Ann Clin Biochem, May 1, 2008; 45(3): 318 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. S. Apple, M. Panteghini, J. Ravkilde, J. Mair, A. H.B. Wu, J. Tate, F. Pagani, R. H. Christenson, A. S. Jaffe, and on Behalf of the Committee on Standardization of M
Quality Specifications for B-Type Natriuretic Peptide Assays
Clin. Chem., March 1, 2005; 51(3): 486 - 493.
[Abstract] [Full Text] [PDF]


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