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Clinical Chemistry 50: 975-976, 2004; 10.1373/clinchem.2003.027102
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(Clinical Chemistry. 2004;50:975-976.)
© 2004 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Proteinase K Added to the Extraction Procedure Markedly Increases RNA Yield from Primary Breast Tumors for Use in Microarray Studies

Suzanne Egyházi1,a, Judith Bjöhle1, Lambert Skoog1, Fei Huang2, Anna-Lena Borg1, Marianne Frostvik Stolt1, Torsten Hägerström1, Ulrik Ringborg1 and Jonas Bergh1

1 Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska University Hospital-Solna, S-171 76 Stockholm, Sweden2 Bristol-Myers Squibb, Princeton, NJ

aAuthor for correspondence. Fax 46-8-5177-4245; e-mail Suzanne.Egyhazi@onkpat.ki.se.

The first 20% of the full text of this article appears below.


To the Editor:

Gene expression profiling by microarray technology has demonstrated the possibilities of biological separation, prognostication, and prediction (1)(2). In this study we demonstrate that the addition of proteinase K to the extraction procedure improves the yield of RNA from primary breast tumors, making the majority of samples eligible for array analysis by Qiagen RNeasy Minikit. In addition, we investigated whether the addition of proteinase K has any influence on the gene expression profile.

The Qiagen RNeasy Mini protocol was used as described by the manufacturer (3)(4) for extraction of total RNA from 209 frozen primary breast tumors from women who had undergone surgery for breast cancer at Karolinska Hospital. The study was approved by an ethics committee at the hospital. Biopsies . . . [Full Text of this Article]




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Interlaboratory Performance of a Microarray-Based Gene Expression Test to Determine Tissue of Origin in Poorly Differentiated and Undifferentiated Cancers
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Quantitative real-time PCR analysis and microarray-based RNA expression of HER2 in relation to outcome
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