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Serial Analysis of Plasma Proteomic Signatures in Pediatric Patients with Severe Acute Respiratory Syndrome and Correlation with Viral Load

¹ Centre for Emerging Infectious Diseases and Departments of² Clinical Oncology,³ Chemical Pathology, and⁴ Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR

^aaddress correspondence to this author at: Department of Clinical Oncology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30-32 Ngan Shing St., Shatin, New Territories, Hong Kong Special Administrative Region, China; e-mail tcwpoon@cuhk.edu.hk

The first 300 words of the full text of this article appear below.

An outbreak of severe acute respiratory syndrome (SARS) has affected 33 countries on five continents, with 8098 suspected and probable cases and 774 deaths. During the SARS epidemic, more than 1700 infected cases have been reported in Hong Kong. The disease is infectious, particularly within the healthcare setting. Early diagnosis is essential to control the spread of SARS by identifying and isolating infected patients (1)(2). Diagnostic tests have been developed that detect either antibodies to or reverse transcription-PCR (RT-PCR) products of the SARS coronavirus (SARS-CoV). SARS-CoV antibodies can be reliably detected only around 20 days after disease onset (3)(4). Quantitative real-time RT-PCR assays, on the other hand, allow the detection of SARS-CoV within the first week of illness. Our recent study showed that detection sensitivity could approach 80% for plasma and serum samples from SARS patients (5)(6). Aside from improving the analytical and clinical sensitivity of the RT-PCR assays, it is also possible to develop new types of assays on the basis of other biological variables and/or technologies. In the past few years, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) has been applied to the discovery of diagnostic proteomic signatures for cancers such as prostate cancer, ovarian cancer, breast cancer, and hepatocellular carcinoma (7)(8). Here we report the presence of plasma proteomic signatures in serial blood samples from eight pediatric patients with SARS and their correlation with SARS-CoV RNA concentrations in plasma (6).

The serial plasma samples investigated in this study were those examined for SARS-CoV RNA by real-time RT-PCR in our recently reported study (6). The pediatric patients were admitted to the New Territories East Cluster of Hospital Authority Hospitals in Hong Kong and satisfied the WHO surveillance . . . [Full Text of this Article]

The following articles in journals at HighWire Press have cited this article:

				R. T.K. Pang, T. C.W. Poon, K.C. A. Chan, N. L.S. Lee, R. W.K. Chiu, Y.-K. Tong, S. S.C. Chim, J. J.Y. Sung, and Y.M. D. Lo Serum amyloid a is not useful in the diagnosis of severe acute respiratory syndrome. Clin. Chem., June 1, 2006; 52(6): 1202 - 1204. [Full Text] [PDF]

				R. T.K. Pang, T. C.W. Poon, K.C. A. Chan, N. L.S. Lee, R. W.K. Chiu, Y.-K. Tong, R. M.Y. Wong, S. S.C. Chim, S. M. Ngai, J. J.Y. Sung, et al. Serum Proteomic Fingerprints of Adult Patients with Severe Acute Respiratory Syndrome Clin. Chem., March 1, 2006; 52(3): 421 - 429. [Abstract] [Full Text] [PDF]

				A M Li and P C Ng Severe acute respiratory syndrome (SARS) in neonates and children Arch. Dis. Child. Fetal Neonatal Ed., November 1, 2005; 90(6): F461 - F465. [Abstract] [Full Text] [PDF]