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Clinical Chemistry 50: 1473-1475, 2004; 10.1373/clinchem.2004.033712
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(Clinical Chemistry. 2004;50:1473-1475.)
© 2004 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Interference by Gelatin-Based Plasma Substitutes in Capillary Zone Electrophoresis

Koenraad Gijbels1, Johan De Coster2 and Xavier Bossuyt1,a

1 Laboratory Medicine, Immunology, and2 Anesthesia, Leuven University Hospitals, Katholieke Universiteit Leuven, Leuven, Belgium

aAddress correspondence to this author at: Leuven University Hospitals, Laboratory Medicine, Herestraat 49, B-3000 Leuven, Belgium. Fax 32-16-34-79-31; e-mail xavier.bossuyt@uz.kuleuven. ac.be.

The first 20% of the full text of this article appears below.


To the Editor:

Analysis of serum proteins by capillary zone electrophoresis (CZE) is used to detect dysproteinemias and monoclonal proteins [reviewed in (1)]. Most interferences in this method are caused by interference with ultraviolet detection [reviewed in (2)]. For example, radio-opaque contrast agents and, to a lesser extent, some antibiotics absorb at 200–214 nm and produce distinct peaks on the electropherogram. A gelatin-based plasma substitute has also been reported to produce an increase in the {gamma}-region in a polyclonal-like way (3). We describe the systematic evaluation of commercially available synthetic colloidal plasma substitutes for their potential to interfere with serum protein analysis by CZE.

WE used the Paragon 2000® (Beckman Coulter) and the Capillarys® (Sebia) CZE systems. The former uses 214 nm and the latter 200 nm as detection wavelength. The Hydrasys/Hyrys/Hydragel® (Sebia) agarose gel electrophoresis (AGE) system was used for comparison.

Synthetic colloidal plasma substitutes can be divided into products based on dextran, starch, or gelatin (4). We studied (a) dextran-based Rheomacrodex® (depolymerized dextran; Mr 40 000; 100 . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
P. Vermeersch, G. Marien, and X. Bossuyt
A Case of Pseudoparaproteinemia on Capillary Zone Electrophoresis Caused by Geloplasma
Clin. Chem., December 1, 2006; 52(12): 2309 - 2311.
[Full Text] [PDF]


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