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Clinical Chemistry 50: 1715-1717, 2004; 10.1373/clinchem.2004.036061
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(Clinical Chemistry. 2004;50:1715-1717.)
© 2004 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Measurements, Zymographic Analysis, and Characterization of Matrix Metalloproteinase-2 and -9 in Healthy Human Umbilical Cord Blood

Ferdinando Mannello1,a, Francesca Luchetti2, Barbara Canonico3, Elisabetta Falcieri2,4 and Stefano Papa2,3

1 Istituto Istologia e Analisi Laboratorio2 Istituto Scienze Morfologiche3 Centro Citometria e Citomorfologia Università Studi "Carlo Bo", Urbino (PU), Italy
4 Istituti Ortopedici Rizzoli-Consiglio Nazionale delle Ricerche (ITOI-CNR), Bologna, Italy

aAddress correspondence to this author at: Istituto Istologia e Analisi Laboratorio, Via E. Zeppi, snc, Università Studi "Carlo Bo", 61029 Urbino (PU), Italy. Fax 39-0722-322370; e-mail f.mannello@uniurb.it.

The first 20% of the full text of this article appears below.


To the Editor:

Matrix metalloproteinases (MMPs) are zinc/calcium-dependent effectors/mediators of physiologic and pathologic reproductive processes (1); MMPs may alter fetal homeostasis and be involved in pathologic syndromes of pregnancy (2). No information is available on MMPs circulating in healthy umbilical cord plasma (UCP). To characterize the effect(s) of umbilical cord blood (UCB) sampling and to identify possible cellular source(s) of MMP-2 (EC 3.4.24.24) and MMP-9 (EC 3.4.24.35) circulating in UCB, we investigated their concentrations, biochemical characteristics, and isoform distributions by immunoassay and gelatin zymography.

We collected UCB (n = 20) immediately after delivery from women with uncomplicated healthy pregnancies; mean (SD) gestation age at delivery was 38 (2) weeks. We used plastic tubes containing lithium heparin, dipotassium EDTA, or buffered sodium citrate (9NC); sera were obtained from clot-activator-gel tubes (SST; Becton Dickinson). After centrifugation at 1000g for 15 min, supernatants were collected. Leukocyte subpopulations were tested for their subsets and gelatinase content after sedimentation on Lympholyte® gradient (Cedarlane) (3).

Umbilical cord vein sections (n = 5) were excised, separated from the Wharton’s jelly, homogenized, and centrifuged at 8000g for 30 min; the supernatants were then analyzed (4).

Gelatinase calibrators were prepared from healthy capillary peripheral blood (. . . [Full Text of this Article]






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