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Clinical Chemistry 51: 242-244, 2005. First published October 28, 2004; 10.1373/clinchem.2004.038125
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(Clinical Chemistry. 2005;51:242-244.)
© 2005 American Association for Clinical Chemistry, Inc.

Technical Briefs

Impact of Formaldehyde on the in Vitro Proportion of Fetal DNA in Maternal Plasma and Serum

Alexandra Benachi1, Amina Yamgnane1, Martine Olivi2, Yves Dumez1, Evelyne Gautier2 and Jean-Marc Costa2,a

1 Maternité, Hôpital Necker-Enfants Malades, AP-HP-Université Paris, Paris, France 2 Centre de Diagnostic Prénatal, American Hospital of Paris, Neuilly, France

aaddress correspondence to this author at: M. Dassault Molecular Biology Laboratory, Centre de Diagnostic Prénatal, American Hospital of Paris, 63 bd Victor Hugo, 92200 Neuilly-sur-Seine, France; e-mail jean-marc.costa@ahparis.org

The first 20% of the full text of this article appears below.

Analysis of cell-free fetal DNA circulating in maternal blood is now well recognized as a useful tool for noninvasive prenatal diagnosis. Determination of fetal sex has modified prenatal diagnosis for women at risk of transmitting X-linked disorders (1), as well as management of pregnancies at risk for congenital adrenal hyperplasia (2). Routine determination of fetal rhesus D status has been evaluated by several groups (3)(4)(5). Despite the increasing number of potential applications, the low proportion of fetal DNA in a high background of maternal DNA dramatically limits its study, although some groups have already reported such analyses (6)(7)(8).

Recently, Dhallan et al. (9) reported that the addition of formaldehyde to maternal blood increased the percentage of fetal DNA recovered. Because of its potential implications, this requires confirmation. We conducted a study similar to that of Dhallan et al. (9) but used an automated procedure for the extraction of nucleic acids and real-time quantitative PCR to provide more precise measurements of DNA.

We recruited 30 pregnant women carrying a male fetus for this study. The mean (range) gestational age was 29 (15–39) weeks. After receipt of informed consent, blood was collected in 5-mL Vacutainer® Tubes with or without (EDTA tube) clot activator, with or without a neutral solution of formaldehyde (1 mL/L final concentration).

Less than 24 h after blood sampling, each EDTA blood was centrifuged at 1600g for 10 min at room temperature, and the plasma was carefully transferred to a new tube, avoiding disruption of the buffy coat. The plasma was again centrifuged in a similar manner and stored at –80 °C until further processing. For tubes containing clot activator, serum . . . [Full Text of this Article]




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Noninvasive prenatal diagnosis of monogenic diseases by digital size selection and relative mutation dosage on DNA in maternal plasma
PNAS, December 16, 2008; 105(50): 19920 - 19925.
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Y. M. D. Lo, F. M. F. Lun, K. C. A. Chan, N. B. Y. Tsui, K. C. Chong, T. K. Lau, T. Y. Leung, B. C. Y. Zee, C. R. Cantor, and R. W. K. Chiu
From the Cover: Digital PCR for the molecular detection of fetal chromosomal aneuploidy
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