Clinical Chemistry
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Clinical Chemistry 51: 2209-2210, 2005; 10.1373/clinchem.2005.056366
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(Clinical Chemistry. 2005;51:2209-2210.)
© 2005 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Comparison of Protocols for Extracting Circulating DNA and RNA from Maternal Plasma

Rossa W.K. Chiu1, Wing-bong Lui1, Ahmad El-Sheikhah2, Anthony T.C. Chan3, Tze K. Lau4, Kypros H. Nicolaides2 and Y.M. Dennis Lo1,a

Departments of1 Chemical Pathology3 Clinical Oncology and4 Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR
2 Harris Birthright Research Centre for Fetal Medicine, King’s College Hospital, London, United Kingdom

aAddress correspondence to this author at: Department of Chemical Pathology, The Chinese University of Hong Kong, Room 38023, 1/F Clinical Sciences Building, Prince of Wales Hospital, 30-32 Ngan Shing St., Shatin, New Territories, Hong Kong Special Administrative Region, China. Fax 852-2194-6171; e-mail loym@cuhk.edu.hk.

The first 20% of the full text of this article appears below.


To the Editor:

We compared 2 column-based and an automated magnetic bead separation protocol for extracting circulating DNA and RNA from maternal plasma. We obtained peripheral blood from pregnant women at Prince of Wales Hospital, Hong Kong, and King’s College Hospital, London, with informed consent and institutional ethics approval.

Thirty second-trimester samples (median gestational age, 17.6 weeks) were divided into 2 aliquots for maternal plasma DNA extraction by either a QIAamp Mini Kit (Qiagen), with 800 µL of plasma applied per column and DNA elution into 50 µL of deionized water, or a MagNA Pure Total Nucleic Acid Large Volume Isolation Kit on the MagNA Pure LC instrument (Roche Diagnostics) (1) with DNA elution into 50 µL of elution buffer. ß-Globin and SRY concentrations were quantified by real-time PCR assays(2).

With 5 µL of plasma DNA per PCR (2) for samples from women with male fetuses, SRY amplification was observed in 15 (100%) column-method samples . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Pathol.Home page
E C W Hung, R W K Chiu, and Y M D Lo
Detection of circulating fetal nucleic acids: a review of methods and applications
J. Clin. Pathol., April 1, 2009; 62(4): 308 - 313.
[Abstract] [Full Text] [PDF]


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