Clinical Chemistry
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Clinical Chemistry 51: 2419-2420, 2005; 10.1373/clinchem.2005.056010
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(Clinical Chemistry. 2005;51:2419-2420.)
© 2005 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Use of an Automated Method Improves the Yield and Quality of Cell-Free Fetal DNA Extracted from Maternal Plasma

Dorothy J. Huang1, Bernhard G. Zimmermann1,2, Wolfgang Holzgreve1 and Sinuhe Hahn1,a

1 University Women’s Hospital, Department of Research, Basel, Switzerland
2 Center for Research in Biomedicine, UWE Bristol Genomics, Research Institute, University of the West of England, Bristol, United Kingdom

aAddress correspondence to this author at: University Women’s Hospital/Department of Research, Spitalstrasse 21, CH 4031 Basel, Switzerland. Fax 41-61-265-9399; e-mail shahn@uhbs.ch.

The first 20% of the full text of this article appears below.


To the Editor:

Cell-free fetal DNA in the maternal circulation holds great potential for noninvasive prenatal diagnosis (1)(2). However, the manual isolation of cell-free DNA from plasma can be a time-consuming process, and current techniques involving spin columns usually require multiple reloadings of the columns with sample material, increasing the risk of contamination (3)(4)(5). Use of an automated system for DNA isolation can reduce the time involved, increase the throughput, and help reduce inconsistencies resulting from human error in processing of different plasma samples. We compared 2 techniques for extracting cell-free DNA from maternal plasma: the MagNA PureTM LC Instrument (Roche Applied Science), an automated DNA isolation system; and a frequently used manual technique involving spin-column technology (High Pure PCR Template Preparation KitTM; Roche Diagnostics).

After approval was granted from the Cantonal Institutional Review Board of Basel, we obtained . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
Y. Li, W. Holzgreve, V. Kiefer, and S. Hahn
MALDI-TOF Mass Spectrometry Compared With Real-Time PCR for Detection of Fetal Cell-Free DNA in Maternal Plasma
Clin. Chem., December 1, 2006; 52(12): 2311 - 2312.
[Full Text] [PDF]


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