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Clinical Chemistry 51: 1070-1071, 2005; 10.1373/clinchem.2005.048215
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(Clinical Chemistry. 2005;51:1070-1071.)
© 2005 American Association for Clinical Chemistry, Inc.

Letters to the Editor

(Micro)Albuminuria: Antigen Excess Detection in the Roche Modular Analyzer

Andries J. Bakkera, Ageeth Bierma-Ram, Halbe Keidel, Haye Syperda and Appie Zijlstra

Department of Clinical Chemistry, Klinisch Chemisch Laboratorium, Leeuwarden, The Netherlands

aAddress correspondence to this author at: Department of Clinical Chemistry, Klinisch Chemisch Laboratorium, PO Box 850, 8901 BR Leeuwarden, The Netherlands. Fax 31-582882227; e-mail a.j.bakker@kcl.znb.nl.

The first 20% of the full text of this article appears below.


To the Editor:

Measurement of urinary albumin is performed both for detection of microalbuminuria (1) and for establishing the selectivity of urinary protein excretion when renal function deteriorates (2); it thus requires assays that can measure albumin concentrations from a few milligrams per liter to several grams per liter. Falsely low concentrations are encountered regularly in immunoturbidimetric assays as a result of antigen excess (3). Procedures to detect antigen excess include additional testing with qualitative measurement of urinary albumin by reagent strip (dipstick) (4)(5)(6), quantitative measurement of urinary total protein (7), or albumin analysis with multiple dilutions of the sample (8). Another procedure uses an excessive amount of antibody, which can lengthen the calibration curve considerably (9). All of these procedures are costly. Mathematical procedures based on the kinetic differences between samples with and without antigen excess have been proposed (9)(10), but they falsely suggest antigen excess in samples with low antigen concentrations. An efficient procedure involves adding . . . [Full Text of this Article]






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