Clinical Chemistry
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Clinical Chemistry 51: 1261-1263, 2005. First published April 28, 2005; 10.1373/clinchem.2005.050666
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(Clinical Chemistry. 2005;51:1261-1263.)
© 2005 American Association for Clinical Chemistry, Inc.

Technical Briefs

Clinical Applications of Plasma Circulating mRNA Analysis in Cases of Gestational Trophoblastic Disease

Hideaki Masuzaki1,1, Kiyonori Miura1,1,a, Kentaro Yamasaki1, Shoko Miura1, Koh-ichiro Yoshiura2, Shuichiro Yoshimura1, Daisuke Nakayama1, Christophe K. Mapendano2, Norio Niikawa2,3 and Tadayuki Ishimaru1

Departments of1 Obstetrics and Gynecology, and 2 Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan;3 CREST, Japan Science and Technology Agency (JST), Kawaguchi, Japan;

aaddress correspondence to this author at: Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan; fax 81-95-849-7365, e-mail kiyonori@net.nagasaki-u.ac.jp

The first 20% of the full text of this article appears below.

The finding of tumor-related mRNA (tyrosinase mRNA, telomerase component mRNA, various tumor-derived mRNAs, and viral RNAs) in plasma from a patient with cancer suggests that real-time quantitative reverse transcription-PCR (RT-PCR) study of circulating plasma mRNAs may be used as a very simple assay for residual tumor after surgery or therapy (1)(2)(3).

Gestational trophoblastic diseases (GTDs) are a heterogeneous group of diseases that include partial and complete hydatidiform moles (CHMs), invasive moles, choriocarcinomas, placental site trophoblastic tumors, and epithelioid trophoblastic tumors. Human chorionic gonadotropin (hCG) is generally used as a tumor marker in the clinical management of all GTDs. Recently, hCG-ß mRNA from placenta has been detected in maternal plasma by real-time quantitative RT-PCR (4), suggesting that this assay may be useful in the management of GTDs.

In this study, we measured plasma mRNA concentrations by real-time RT-PCR in 3 GTD patients with CHMs and 1 with choriocarcinoma.

Blood samples (10 mL) were collected before and after suction evacuation or chemotherapy. Plasma mRNA was extracted, and a 1-step real-time RT-PCR assay was performed as described by Ng et al. (4). We selected . . . [Full Text of this Article]






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