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Clinical Chemistry 51: 1313-1314, 2005; 10.1373/clinchem.2004.043976
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(Clinical Chemistry. 2005;51:1313-1314.)
© 2005 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Mutations in K-ras Codon 12 Detected in Plasma DNA Are Not an Indicator of Disease in Patients with Non-Small Cell Lung Cancer

Sonya Trombino1, Monica Neri2, Riccardo Puntoni2, Cristiano Angelini1, Maura Loprevite3, Alfredo Cesario4,9, Pierluigi Granone4, Andrea Imperatori5, Lorenzo Dominioni5, Andrea Ardizzoni6, Rosangela Filiberti7 and Patrizia Russo8,a

1 Department of Biology, University of Genoa, Genoa, Italy
2 Unit of Epidemiology and Biostatistics, 3 Unit of Medical Oncology A, 7 Unit of Molecular Epidemiology, and, 8 Unit of Translational Research B (Lung Cancer), National Cancer Institute, Genoa, Italy
4 General Thoracic Surgery, Catholic University, Rome, Italy
5 Unit of Thoracic Surgery, Insubria University, Varese, Italy
6 Unit of Medical Oncology, Hospital of Parma, Parma, Italy
9 Translational & Clinical Respiratory Pathology Laboratory IRCCS, San Raffaele, Rome, Italy

aAddress correspondence to this author at: Translational Research B (Lung Cancer), Department of Integrated Medical Oncology (DOMI), National Cancer Institute, Largo Rosanna Benzi 10, I-16132 Genoa, Italy. Fax 39-010-5600217; e-mail patrizia.russo@istge.it.

The first 20% of the full text of this article appears below.


To the Editor:

The demonstration that cell-free circulating DNA detected in the plasma of cancer patients is genetically identical to that of the primary tumor has generated substantial interest, leading to >200 publications (http://www.ncbi.nlm.nih.gov). Recently, Wang et al. (1) reported in this journal that the method chosen for DNA isolation might contribute significantly to mutation detection (in their case, K-ras mutations in the plasma of patients with colorectal cancer). Briefly, they recommended the use of a modified guanidine/Promega resin method (G/R) to isolate DNA, affirming that this method enhances assay sensitivity. We used the same approach to detect K-ras mutations in the plasma of patients with non-small cell lung cancer (NSCLC) and compared the Qiagen vs the G/R method for isolation of circulating DNA. We purified DNA from plasma samples and cancer tissues from 12 patients. The DNA in 2 aliquots of the plasma from each patient was isolated by the Qiagen method . . . [Full Text of this Article]




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