Clinical Chemistry Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 51: 1738-1742, 2005; 10.1373/clinchem.2005.050211
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Flanders, M. M.
Right arrow Articles by Rodgers, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Flanders, M. M.
Right arrow Articles by Rodgers, G. M.
Related Collections
Right arrow Laboratory Management
Right arrow Hemostasis and Thrombosis
(Clinical Chemistry. 2005;51:1738-1742.)
© 2005 American Association for Clinical Chemistry, Inc.

Technical Briefs

Pediatric Reference Intervals for Seven Common Coagulation Assays

Michele M. Flanders1, Ronda A. Crist2, William L. Roberts1,3 and George M. Rodgers1,3,4,a

1 ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT;2 ARUP Laboratories, Salt Lake City, UT;3 Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT;4 Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, UT;

aaddress correspondence to this author at: Division of Hematology, University of Utah Health Sciences Center, 50 North Medical Dr., Salt Lake City, UT 84132; fax 801-585-5469, e-mail george.rodgers@hsc.utah.edu

The first 300 words of the full text of this article appear below.

Accurate interpretation of pediatric coagulation tests is complicated by the fact that reference intervals for many assays differ from those for adults. In 1992, Andrew et al. (1) reported childhood coagulation reference intervals for ages 1–5, 6–10, and 11–16 years with a minimum of 4 and maximum of 7 individuals at each age, with 20–50 per age group. These results have served as the basis for most pediatric coagulation reference intervals for over a decade. However, with the development of newer reagents, methodologies, and instruments to measure coagulation analytes, results from this study, which was limited by its small size, may be less relevant.

In 2002, we initiated a project to collect blood and urine samples from healthy children 7–17 years of age, with the goal of establishing pediatric reference intervals for many laboratory tests (2). The purpose of this study was to determine pediatric reference intervals for 7 coagulation tests associated with common bleeding disorders.

Samples were drawn for reference interval studies from 902 healthy children, ages 7–17 years. All were healthy, had no history of bleeding or thrombotic disorders, and were taking no medications for at least 2 weeks before specimen collection. Informed consent was obtained from parents, and the study was approved by the University of Utah Institutional Review Board. Samples used to establish adult reference intervals were purchased from George King Bio-Medical or Precision Biologic, or were collected from local volunteers.

Blood was obtained by clean venipuncture; a pilot tube was drawn first. An exact ratio of 9 volumes of blood to 1 volume of anticoagulant (32 g/L citrate) was maintained. Specimens were centrifuged immediately at 3000g for 20 min at room temperature, aliquoted, and frozen at –80 °C.

Prothrombin time (PT); partial thromboplastin time (PTT); factors VIII, IX, and XI; . . . [Full Text of this Article]






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the American Association for Clinical Chemistry.