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Clinical Chemistry 53: 2221-2222, 2007; 10.1373/clinchem.2007.095406
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(Clinical Chemistry. 2007;53:2221-2222.)
© 2007 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Ammonium 5-Bromo-7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate: A New Fluorogenic Reagent for the Determination of Aminothiols by HPLC

Giorgio Cevascoa, Anna Maria Mumot, Carlo Scapolla and Sergio Thea

Dipartimento di Chimica e, Chimica Industriale Università, di Genova and Consiglio, Nazionale delle Ricerche, Genova, Italia

aAddress correspondence to this author at: Dipartimento di Chimica e Chimica Industriale, Consiglio Nazionale delle Ricerche, Via Dodecaneso, 31 I-16146 Genova, Italy. Fax (+39)010 353 6107; e-mail giocev@chimica.unige.it.

The first 20% of the full text of this article appears below.


To the Editor:

The measurement of aminothiols such as cysteine (Cys), cysteinyl-glycine (Cys-Gly), homocysteine (Hcy), and glutathione (GSH), as well as the corresponding disulfides, has gained high interest within the biomedical community because such molecules are important biomarkers for a wide range of diseases. In particular, increased total plasma Hcy is now considered a risk factor for cardiovascular disorders(1) as well as other degenerative conditions. Although various methods for the measurement of aminothiols are available, HPLC coupled with fluorometric detection is one of the most suitable techniques for determination of minute amounts of thiols(2). Most methods require precolumn derivatization, and although different types of fluorogenic reagents for thiols have been proposed, the most commonly used and sensitive reagent is the commercially available ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F)(3).

With this method the completion of the reaction takes a very long time and the conditions required are quite strict, i.e., derivatization must be carried out at 60 °C for a 1-h period in a moderately alkaline medium (pH = 9.5) in the . . . [Full Text of this Article]






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Copyright © 2007 by the American Association for Clinical Chemistry.