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Clinical Chemistry 54: 451-452, 2008; 10.1373/clinchem.2007.096594
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(Clinical Chemistry. 2008;54:451-452.)
© 2008 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Measurement of Creatinine in Whole Blood Samples Supplemented to Achieve Increased Creatinine Concentrations

Paul D’Orazioa, JoAnn Conant and Jose Cervera

Instrumentation Laboratory Lexington, Massachusetts 02421

aAddress correspondence to this author at: Instrumentation Laboratory, 101 Hartwell Avenue, Lexington, Massachusetts 02421, Fax (781) 861-4452, e-mail pdorazio@ilww.com

The first 20% of the full text of this article appears below.


To the Editor:

During clinical trials of a new biosensor for measurement of whole blood creatinine, we were unable to obtain sufficient quantities of samples with naturally occurring high creatinine concentrations to validate performance of the sensor across the proposed reportable concentration range for the device [18–1326 µmol/L (0.2–15.0 mg/dL)]. Addition of creatinine to whole blood samples was required to validate performance of the sensor at the high end of the range. We noticed irreproducible results for samples that were supplemented with creatinine to increase the concentration. Like other direct-reading electrochemical biosensors (1), creatinine sensors respond to the molality of creatinine in the sample (amount of creatinine per unit mass of water). It is known that molality of creatinine in erythrocyte fluid is equal to molality in plasma (2) and that creatinine is transported by passive diffusion through the lipid bilayer of the erythrocyte membrane (3).

Heparinized blood from a healthy volunteer was centrifuged and the separated plasma supplemented . . . [Full Text of this Article]






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