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Clinical Chemistry 54: 925-927, 2008; 10.1373/clinchem.2007.096628
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(Clinical Chemistry. 2008;54:925-927.)
© 2008 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Agreement of Different Immunoassays for Urinary Albumin Measurement

Joíza L. Camargo1,a, Gustavo M. Lara2, Andréa E. Wendland1, Jorge L. Gross3 and Mirela J. de Azevedo3

1 Clinical Pathology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil
2 FEEVALE University Centre, Novo Hamburgo, Brazil
3 Endocrinology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil

aAddress correspondence to this author at: Serviço de Patologia Clínica, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcellos, 2350, 2° andar, Porto Alegre, RS, 90035-903, Brazil, Fax +55-51-33325188, e-mail jcamargo@hcpa.ufrgs.br

The first 20% of the full text of this article appears below.


To the Editor:

Diagnosis of diabetic nephropathy (DN) is based on measurements of urinary albumin (UALB) by a sensitive analytical method with sufficient analytical precision (CV <15%) to detect changes in UALB concentration(1)(2)(3). We evaluated the comparative diagnostic accuracies of 4 UALB assays for DN.

We used urine samples from 98 diabetic outpatients. UALB was measured by 4 immunoturbidimetric (IT) assays: the Urine Pack Immuno method (Bayer) as implemented on a Roche Cobas Mira Plus (method A), Malb Aptec Diagnostic (BioSys) (method B), Albumin Tina-quant (Roche) (method C), and Microalbumin (Randox) (method D). Methods B, C, and D were implemented on a Hitachi 917 analyzer (Roche). Method A was considered as the reference standard (comparison method) because it had been used in our laboratory since 1996 and validated in previous studies(2)(4)(5). Total urinary protein was measured before UALB, and the U/CSF Protein assay (Roche) on the Hitachi 917 analyzer (Roche) was used to estimate the contribution (%) of albumin to total protein for each . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Nephrol Dial TransplantHome page
C. K. Kramer, J. Camargo, E. D. Ricardo, F. K. Almeida, L. H. Canani, J. L. Gross, and M. J. Azevedo
Does bacteriuria interfere with albuminuria measurements of patients with diabetes?
Nephrol. Dial. Transplant., November 17, 2008; (2008) gfn629v1.
[Abstract] [Full Text] [PDF]


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