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Clinical Chemistry 54: 937-939, 2008; 10.1373/clinchem.2008.104067
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(Clinical Chemistry. 2008;54:937-939.)
© 2008 American Association for Clinical Chemistry, Inc.

Editorial

Protein Microarrays Address the Elephant in the Room

Michael G. Kattah1, Paul J. Utz1 and Imelda Balboni1,2,a

1 Department of Medicine, Division of Immunology and Rheumatology, Stanford University, Stanford, CA
2 Department of Pediatrics, Division of Rheumatology, Stanford University, Stanford, CA

aAddress correspondence to this author at: Stanford University, Department of Medicine, 269 Campus Dr. CCSR 2250, Stanford, CA 94305, Fax 650-723-7509, E-mail mbalboni@stanford.edu

The first 20% of the full text of this article appears below.

Protein arrays, antigen microarrays in particular, are powerful tools for interrogating the humoral immune response in the setting of health and disease. Whereas antibodies against pathogen-associated antigens provide defense against infection, autoantibodies target a wide variety of cell surface, cytoplasmic, and nuclear self-antigens. Instead of analyzing these antibodies one at a time in single-analyte experiments, investigators can now perform multiplexed profiling of antibody reactivities to a panel of antigens spotted onto planar antigen microarrays(1)(2). Since their inception, antigen microarrays have been used to guide DNA vaccine therapy(3), to profile antibody reactivity during viral infections(4), and to identify clinical subtypes of rheumatoid arthritis(5), lupus(6), and prostate cancer(7). Despite recent advances, the need for improvement in antigen microarrays is still tremendous. Some of the unique challenges facing antigen microarrays include technical issues regarding printing and probing, normalization strategies, and the lack of a reference sample for standardization between experiments and laboratories. Another issue facing antigen microarrays, and protein arrays in general, is that proteins exist at widely different concentrations, and the simultaneous measurement of both low-abundance and high-abundance proteins represents a technical challenge. In this issue of Clinical Chemistry, Hartmann et al.(8) describe a strategy for accurately measuring total immunoglobulin, a high-abundance protein, while . . . [Full Text of this Article]




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Home page
 Clin. Chem.Home page
J. Gordon
Protein Microarrays: Before the Elephant Got in the Room
Clin. Chem., December 1, 2008; 54(12): 2087 - 2088.
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