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Clinical Chemistry 45: 1313, 1999;
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(Clinical Chemistry. 1999;45:1313.)
© 1999 American Association for Clinical Chemistry, Inc.

Letters

Technique to Remove Interference Caused by Radio-Opaque Agents in Clinical Capillary Zone Electrophoresis

Cynthia R. Blessuma

a Author for correspondence. Fax 714-961-3759; e-mail crblessum{at}beckman.com

Neeta Khatter and Stephen C. Alter

Beckman Coulter, Inc., 200 S. Kraemer Blvd., P.O. Box 8000, Brea, CA 92822-8000


To the Editor:

We read with great interest the report "Interference of Radio-opaque Agents in Clinical Capillary Zone Electrophoresis" (1). A laboratory sent two similar patient samples for our evaluation. Both patients had received intravenous sodium meglumine ioxitalamate (Telebrix), a radio-opaque contrast material that absorbs at 214 nm. The interfering substance was not visible on agarose gels (Paragon® SPE), whereas the capillary zone electrophoresis (CZE) results (Paragon CZE® 2000) showed a peak in the alpha-2 zone, which may be mistaken for a monoclonal protein (see Fig. 1 A). The anomalous peak was not removed by reaction with antisera to any of the immunoglobulins.



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  		Figure 1. CZE electropherograms of a patient serum sample before (A) and after (B) desalting.

(A), the peak for the radio-opaque agent is indicated by the arrow.

The samples were then desalted (D-Salt Dextran plastic desalting columns, 5-kDa cutoff; Pierce). Several similar products are available. After capillary electrophoresis, the desalted samples (see Fig. 1BUp ) appeared normal with the questionable peak entirely removed, thus confirming that the interfering substance was of low molecular weight and, therefore, not a paraprotein. The desalting procedure was fast and simple. Clinical laboratories that use CZE could easily use this method to evaluate questionable samples.


Acknowledgments

This information was presented previously as part of a technical poster, "Detection of Non-Protein Peaks in Serum by Capillary Zone Electrophoresis", by C. Blessum, M. Andres, and N. Khatter, at the 12th International Symposium on Capillary Electrophoresis and Related Microscale Techniques, January 23–28, 1999, in Palm Springs, CA.


References

  1. Bossuyt X, Mewis A, Blanckaert N. Interference of radio-opaque agents in clinical capillary zone electrophoresis. Clin Chem 1999;45:129-131. [Free Full Text]



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This Article
Right arrow Extract Freely available
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blessum, C. R.
Right arrow Articles by Alter, S. C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Blessum, C. R.
Right arrow Articles by Alter, S. C.
Related Collections
Right arrow Proteomics and Protein Markers
Right arrow Automation and Analytical Techniques

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