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Clinical Chemistry 46: 308-309, 2000;
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(Clinical Chemistry. 2000;46:308-309.)
© 2000 American Association for Clinical Chemistry, Inc.

Letters

Correction for Stott et al.: Simple Multiplex PCR for the Simultaneous Detection of the C282Y and H63D Hemochromatosis (HFE) Gene Mutations

Marion K. Stott

a Author for correspondence. Fax 03-364-1460.

Andrew P. Fellowes
Jeff D. Upton
Michael J. Burt
Peter M. Georgea

Clinical Biochemistry Unit, Canterbury Health Laboratories, P.O. Box 151, Christchurch, New Zealand


To the Editor:

It has been drawn to our attention that the PCR primer 282rev described in our Technical Brief [M.K. Stott, A.P. Fellowes, J.D. Upton, M.J. Burt, and P.M. George. Simple multiplex PCR for the simultaneous detection of the C282Y and H63D hemochromatosis (HFE) gene mutations. Clin Chem 1999;45:426–8] contains a mismatch, at the penultimate base, to the GenBank sequence (GI: 1890179 at www.ncbi.nlm.nih.gov). This is attributable to an inadvertent error during the design of the assay. The primer sequence described has been used successfully for the analysis of many clinical samples. The primer sequence described introduces an A:A mismatch, which is not particularly destabilizing and does not prevent amplification. Although other groups may prefer to use a perfectly matched "correct" 282rev primer (CCA TCC CCT AAC AAA GAG CAG ATC CTC), this may require reoptimization of the primer concentration and PCR conditions.





This Article
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the Editor about this paper
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Right arrow Alert me when eLetters are posted
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Citing Articles
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Google Scholar
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PubMed
Right arrow PubMed Citation
Right arrow Articles by Stott, M. K.
Right arrow Articles by George, P. M.

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