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Clinical Chemistry 53: 505-510, 2007. First published January 26, 2007; 10.1373/clinchem.2006.080051
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(Clinical Chemistry. 2007;53:505-510.)
© 2007 American Association for Clinical Chemistry, Inc.

Clinical Immunology

Laboratory Diagnosis of Specific Antibody Deficiency to Pneumococcal Capsular Polysaccharide Antigens

Axel Jeurissen1,1, Leen Moens1,1, Marc Raes2, Greet Wuyts1, Luc Willebrords1, Kate Sauer2, Marijke Proesmans2, Jan L. Ceuppens3, Kris De Boeck2 and Xavier Bossuyt1,a

1 Laboratory Medicine, Immunology, University Hospital Leuven, Belgium.
2 Department of Pediatrics, University Hospital Leuven, Belgium.
3 Department of Internal Medicine, Allergy, University Hospital Leuven, Belgium.

aAddress correspondence to this author at: Laboratory Medicine, Immunology, GHB-Herestraat 49, B-3000 Leuven, Belgium. Fax 32-16-34-79-31; e-mail Xavier.bossuyt{at}uz.kuleuven.ac.be.

Background: Measurement of postimmunization antibody response to pneumococcal capsular polysaccharide (caps-PS) is the standard method to identify deficiency of antipolysaccharide antibody production. However, no standardized criteria have been defined for classification of patients into responders or nonresponders to caps-PS.

Methods: We vaccinated 37 healthy children and 39 healthy adults with Pneumovax® and measured the anti–caps-PS antibody response to 5 serotypes. We also measured antipneumococcal antibody titers in 82 patients with increased susceptibility to airway infection. The ELISA was performed according to the 3rd-generation assay format.

Results: The lower 5th percentile (cutoff) concentrations for the postimmunization antibody titer in healthy individuals were 0.67 mg/L, 0.45 mg/L, 0.46 mg/L, 0.31 mg/L, and 1.04 mg/L for serotypes 3, 4, 9N, 18C, and 19F, respectively. In 96% of healthy individuals, antibody responses higher than the cutoff concentration were seen for at least 3 of the 5 serotypes. Nine of 82 patients (11%) failed to mount an adequate antibody response for at least 4 of the 5 serotypes tested, whereas only 1 control (1.3%) failed to do so.

Conclusion: The cutoffs for antibody responses to caps-PS identified in this study appear useful for identifying individuals with an inadequate response to vaccine.




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Immunol.Home page
L. Moens, M. Wuyts, I. Meyts, K. De Boeck, and X. Bossuyt
Human Memory B Lymphocyte Subsets Fulfill Distinct Roles in the Anti-Polysaccharide and Anti-Protein Immune Response
J. Immunol., October 15, 2008; 181(8): 5306 - 5312.
[Abstract] [Full Text] [PDF]


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